Simultaneous analysis of multiple analytes from a single sample, known as multimodal sequencing, is gaining significance as tumor profiling and biomarker research continue to advance at a rapid pace. However, challenges such as limited sample availability, complex workflows, lengthy turnaround times and the substantial investment needed to implement multiple workflows underscore the pressing need for streamlined workflows and assay consolidation.
To address these challenges, our QIAseq multimodal sequencing kits utilize total nucleic acids (DNA and RNA) as input, enabling the creation of UDI-labeled, Illumina-compatible targeted DNA and RNA libraries from as little as 10 ng of input material – all within a single-day, streamlined workflow. By integrating multiple workflows into one, multimodal sequencing simplifies comprehensive profiling by combining the analysis of various analytes into a single DNA and RNA library preparation process. This approach significantly reduces overall costs and cuts the sample-to-sequencing turnaround time from the traditional three days to just one.